Hangzhou Jianyang Information Technology Co. LTD
5 1

Rat Heparan Sulfate Proteoglycan (HSPG)ELISA Kit

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Hangzhou Jianyang Information Technology Co. LTD
[China]

Address
Yuhang Qu Hangzhou Zhejiang
Phone
86-1862688-1068
Contact name
Zhang Jiao

Quick Information

  • Brand Name: Bioswamp
  • Place of Origin: China
  • CAS No. : RA21423
  • :

Description

The kit uses a double antibody sandwich assay to determine the level of rat heparin sulfate proteoglycan (HSPG) in the specimen. The microplate was coated with purified rat heparan sulfate proteoglycan (HSPG) antibody to prepare a solid phase antibody, and heparin sulfate proteoglycan (HSPG) was sequentially added to the microcapsule of the coated monoclonal antibody, and then labeled with HRP. Heparin sulfate proteoglycan (HSPG) binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with heparan sulfate proteoglycan (HSPG) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat heparan sulfate proteoglycan (HSPG) in the sample was calculated from a standard curve.


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